ECTS: 2.8
Course leader: Marianne Hokland
Language: English
Graduate school: Faculty of Health
Graduate program: BIO
Course fee: 3,360.00 DKK
Status: Course is finished
Semester: Spring 2022
Application deadline: 17/01/2022
Cancellation deadline: 27/01/2022
Course type: Classroom teaching
Start date: 31/01/2022
Administrator: Christina Bak Bekhøi Christensen
Title: Advanced Flow Cytometry
Reg.no: B299/02 is being offered by the Graduate School of Health, Aarhus University, spring 2022
Criteria for participation: University degree in medicine, dentistry, nursing, or Master’s degree in other fields and/or postgraduate research fellows (PhD students and research-year medical students).
Aim: The course provides detailed knowledge of advanced flow cytometry, focusing on state-of-the-art flow cytometry methods, and includes extensive practical hands-on data analysis exercises that covers the most important topics and pitfalls. Thus, the course will provide the participant with a broad understanding of how to design multicolor flow cytometry experiments, how to handle sample material, and how to analyze and present flow cytometry data for publication.
Learning outcomes: The students will learn
- (i) how to select the optimal flow cytometry related methodology.
- (ii) how to design and optimize a flow cytometry experiment.
- (iii) which controls to include.
- (iv) how to analyze flow cytometry data including critically evaluation of the experimental results.
- (v) how to present flow cytometry data for publication.
Content:
- Presentation of
- o different flow cytometry related applications within biomedical research.
- o quality controls, sources of error, validation of different flow cytometry methodologies.
- o optimal panel and experimental design.
- o analysis and presentation of flow cytometry data high-dimensional data (e.g. tSNE).
- Practical exercises (hands-on data analysis).
- o titration experiments and compensation.
- o quality controls and validation on instrument performance.
- o analysis and presentation of flow cytometry data, including for publication.
- o high-dimensional data analysis using FlowJo (incl tSNE).
Recommended knowledge for participation: Some degree of practical flow cytometry experience, completion of the PhD course in Basic Flow Cytrometry or comparable knowledge on basic principles in flow cytometry.
Language: English
ECTS: 2.8
Head of course: Marianne Hokland together with Morten Nørgaard Andersen
Instructors: Marianne Hokland, Morten Nørgaard Andersen, Anders Etzerodt, Kasper Lauridsen, Timothy Bushnell, and staff experienced in flow cytometry from the Dept Biomedicine (AU) and Dept Hematology (AUH).
Number of participants: 16
Time and date: 31 January + 1, 2, 3 February 2022
Place:
31/01/2022, Preben Hornungstuen, The Conference Centre, Fredrik Nielsens Vej 4, 8000 Aarhus C
01/02/2022, Preben Hornungstuen, The Conference Centre, Fredrik Nielsens Vej 4, 8000 Aarhus C
02/02/2022, Meetingroom 2, 2.2 and Faculty Club, The Conference Centre, Fredrik Nielsens Vej 4, 8000 Aarhus C
03/02/2022, Preben Hornungstuen, The Conference Centre, Fredrik Nielsens Vej 4, 8000 Aarhus C
Course fee: DKK 3360
Participation in the course is without cost for:
- PhD students, Research Year students and Research Honours Programme students from Aarhus University
- PhD students enrolled at partner universities of the Nordoc collaboration
- PhD students from other institutions in the open market agreement for PhD courses
Course dates:
- 31 January 2022 09:15 - 16:00
- 01 February 2022 09:15 - 16:00
- 02 February 2022 09:15 - 16:00
- 03 February 2022 09:15 - 16:00