ECTS: 3.4
Course leader: Yonglun Luo
Language: English
Graduate school: Faculty of Health
Graduate program: BIO
Course fee: 4,080.00 DKK
Status: Course is finished
Semester: Spring 2021
Application deadline: 01/02/2021
Cancellation deadline: 15/02/2021
Course type: Classroom teaching
Start date: 01/03/2021
Administrator: Annette Poulsen
Course changed to online
Please notice due to the COVID-19 restriction the course has been changed to online participation.
Title: Genome engineering
Reg.nr: B244/06
is being offered by the Graduate School of Health, Aarhus University, spring 2021.
Criteria for participation: University degree in medicine, dentistry, nursing, or Master’s degree in other fields and/or postgraduate research fellows (PhD students and research-year medical students).
Aim: The aim of the course is to:
- Provide students with the state-of-the-art knowledge on CRISPR-Cas9 technology
- Introduce the different types of CRISPR gene editing tools and their applications
- Instruct how to design the best CRISPR gRNA with high on-target efficiency and low off-target effect
- Instruct how to design CRISPR and donor vectors for gene knockin, tagging
- Introduce CRISPR data science and high-throughput CRISPR computing
- Instruct students how to practically analyze and understand CRISPR editing data with TIDE, ICE and NGS
- Introduce the current progress in CRISPR gene therapy
- Prepare PhD students with essential knowledge and tools for their gene editing projects
Learning outcomes: With the completion of this Genome Engineering course, PhD students are expected to
- Acquire a systematic knowledge on CRISPR gene editing technology, progresses, and applications
- Know how to design the CRISPR gRNA for gene knockout
- Learn how to design the donors for gene knockin
- Independently select the best CRISPR system and strategy for a gene editing project
- Independently analyze and interpret CRISPR gene editing results
- Know the concept, resources and potential of CRISPR data science
- Understand the progresses, but also remaining challenges, in CRISPR gene therapy
Contents: This course covers:
- The principle of CRISPR gene editing
- The different genetic manipulation tools derived from CRISPR-Cas9
- Base editing
- Prime editing
- Methods used for quantifying CRISPR editing efficiency
- Ways of delivery CRISPR vectors in vitro and/or in vivo: plasmid, RNP, lentivirus, AAV
- CRISPR data science – on-target activity and off-target effect prediction
- Group teaching on CRISPR vector design for gRNA and donor design for gene knockout, knockin, tagging and single nucleotide mutagenesis
- Group teaching on data analysis
- Introduction to CRISPR gene therapy
Recommended knowledge for participation: Genetics, molecular biology, cell biology, and basic bioinformatics.
Instructors: Jan Gorodkin (KU). Rasmus O. Bak (AU), Ernst-Martin Füchtbauer (AU), Jacob Giehm Mikkelsen (AU), Lin.Lin (AU)
Number of participants: Max. 24, which will be divided into 4 groups in group teachings (in silico CRISPR/Cas9 design)
Dates and times: 1-4 March, 2021 from approx. 9-16 every day.
Place: Online teaching
Participation in the course is without cost for:
- PhD students, Research Year students and Research Honours Programme students from Aarhus University
- PhD students enrolled at partner universities of the Nordoc collaboration
- PhD students from other institutions in the open market agreement for PhD courses
Course dates:
- 01 March 2021 09:00 - 16:00
- 02 March 2021 09:00 - 16:00
- 03 March 2021 09:00 - 16:00
- 04 March 2021 09:00 - 16:00