Course Proteomics and protein profiling


Course leader: Johan Palmfeldt

Language: English

Graduate school: Faculty of Health

Status: Course is finished

Semester: Spring 2016

Application deadline: 01/02/2016

Cancellation deadline: 15/02/2016

Start date: 29/02/2016

Administrator: Charlotte Andersen

142-10/08 Proteomics and protein profiling

is being offered by the Graduate School of Health Sciences, University of Aarhus, spring 2016.

Criteria for participation
: University degree in medicine, dentistry, nursing, or Master’s degree in other fields and/or postgraduate research fellows (PhD students and research-year medical students).

The aim is to give the students an overview of proteomics and the study of complex protein samples. The course will cover the theoretical basis and practical methodology of proteomics. The course attendants will learn how to design and perform proteomic studies and will get understanding of its impact on research within life science and medicine.

The four-day course will start with the basics of protein chemistry and will then cover large scale proteomics and how it can be used to elucidate physiological phenomena and to discover protein biomarkers. The course contains lectures, laboratory experiments, computer exercises individual project and journal club. The focus will be on protein profiling of complex mixtures of proteins and the course covers the steps; sample collection, protein and peptide separation techniques (gel based and chromatography based methods), mass spectrometry analysis as well as data evaluation. Examples will foremost be from clinical protein research.

Learning outcome:

  1. Understanding of the principles of proteomics methodologies, and how the different methods can be combined.
  2. Knowledge of the value of protein analysis in biomedical research, including advantages and limitations.
  3. Acquired practical experience in 1) lab work in protein analysis and 2) software tools used to analyze proteomics data with regard to MS spectra, protein modifications, pathway analysis etc.
  4. To be able to interpret, discuss and critically assess proteomics data.
  5. To be able to design studies on protein analysis and proteomics (type of sample, procedures, instruments etc.).

Course leader:
Johan Palmfeldt, Research Unit for Molecular Medicine at Aarhus University Hospital/Aarhus University.

Staff members of Research Unit for Molecular Medicine, Aarhus University Hospital, Aarhus University, with contribution from invited lectures.


Number of participants:

February 29 - March 3, 2016

Research Unit for Molecular Medicine, Science Center Skejby

Course fee:
DKK 3,600,-

Participation in the course is without cost for:

  • PhD students and research-year students from Aarhus University
  • PhD students from other institutions in the open marked agreement for PhD courses

January 4, 2016


Course dates:

  • 29 February 2016