Course Introduction to Fluorescence Microscopy

ECTS: 2.7

Course leader: Lene Niemann Nejsum

Language: English

Graduate school: Faculty of Health

Graduate program: ClinFO

Course fee: 3,240.00 DKK

Status: Course is open for application

Semester: Spring 2027

Application deadline: 15/12/2026

Cancellation deadline: 03/01/2027

Course type: Classroom teaching

Start date: 12/01/2027

Day 4 and 5 are special

Please note that there will only be 2 hours per student on either day 4 or 5 (19 and 20 January); it is not two whole days of teaching! Times for individual students will be decided on the first course day .

The course C267/10 Introduction to Fluorescence Microscopy is being offered by the Graduate School of Health, Aarhus University, 2027.

Criteria for participation: 
University degree in medicine, dentistry, nursing, or Master’s degree in other fields and/or postgraduate research fellows (PhD students and research-year medical students).

Requirements for participation:
None.

Aim: 
The aim is to give the participants an overview of basic fluorescence microscopy techniques, sample preparation, image acquisition and imaging analysis.

Learning outcomes: 
The students will obtain basic knowledge of different fluorescence microscopy techniques, sample preparation, image acquisition and image analysis. This should enable students to prepare samples for fluorescence microscopy, choose the appropriate microscope setup, acquire images, analyze images and generate publication figures. This will enable students to participate in research projects involving fluorescence microscopy.

Workload:
The full workload of the course is expected to be 26.5 hours

Content: 
Overview of basic light and fluorescence microscopy methods including white light microscopy, wide-field fluorescence microscopy and confocal microscopy as well as optics, cameras and photodetectors.

  • Introduction to sample preparation of cells and paraffin embedded tissue sections for fluorescence microscopy (hands on).
  • Introduction to image analysis using ImageJ Fiji; this includes working with multicolor images, background handling and fluorescence quantification (hands on) and
  • Practical demonstration of wide-field fluorescence image acquisition using an inverted, automated microscope.

 

Instructors: 
Senior Researcher Frédéric H. Login, PhD students Amalie Grønning, Lærke Marcussen,

Venue: 
Bartholin Læsesal and adjacent laboratories. Forum AUH.

Participation in the course is without cost for:

Course dates:

  • 12 January 2027 08:30 - 16:00
  • 13 January 2027 08:30 - 16:00
  • 14 January 2027 08:30 - 16:00
  • 19 January 2027 08:30 - 16:00
  • 20 January 2027 08:30 - 16:00