Course Introduction to Fluorescence Microscopy

The course C267/08 Introduction to Fluorescence Microscopy is being offered by the Graduate School of Health, Aarhus University, 2025.

Criteria for participation: University degree in medicine, dentistry, nursing, or Master’s degree in other fields and/or postgraduate research fellows (PhD students and research-year medical students).

Requirements for participation:
Admissions are selected based on written applications which must be half a page written stating your previous experience with fluorescence microscopy (if any) and how you plan to apply fluorescence microscopy in your PhD studies.

 Please note, that everyone will be placed on waiting list and the seats will be given upon the approval of your application by the course leader.

Aim: 
The aim is to give the participants an overview of basic fluorescence microscopy techniques, sample preparation, image acquisition and imaging analysis.

Learning outcomes: 
The students will obtain basic knowledge of different fluorescence microscopy techniques, sample preparation, image acquisition and image analysis. This should enable students to prepare samples for fluorescence microscopy, choose the appropriate microscope setup, acquire images, analyze images and generate publication figures. This will enable students to participate in research projects involving fluorescence microscopy.

Workload: The full workload of the course is expected to be 39 hours

Content:

• Overview of basic light and fluorescence microscopy methods including white light microscopy, wide-field fluorescence microscopy and confocal microscopy as well as optics, cameras and photodetectors.
• Introduction to sample preparation of cells and paraffin embedded tissue sections for fluorescence microscopy (hands on).
• Introduction to image analysis using ImageJ Fiji; this includes working with multicolor images, background handling and fluorescence quantification (hands on) and
• Practical demonstration of wide-field fluorescence image acquisition using an inverted, automated microscope.
• There will be a mandatory home assignment equivalent to 6 h. 

Language:
English

Head of course:
Lene Niemann Nejsum

Instructors: 
Senior Researcher Frédéric H. Login, PhD students Amalie Grønning, Lærke Marcussen, Benjmain Bach Green, Teresa Kirkegaard and Saba Molhemi 

Time and date:

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Note, on the 21-22/1, it will only be 2 h per student.

Venue:
Bartholin Læsesal and adjacent laboratories. Forum AUH.

Participation in the course is without cost for:

• PhD students, Health Research Year students from Aarhus University
• PhD students enrolled at partner universities of the Nordoc collaboration
• PhD students from other institutions in the open market agreement for PhD courses