Course Genome Engineering

ECTS: 3.5

Course leader: Yonglun Luo

Language: English

Graduate school: Faculty of Health

Graduate program: BIO

Course fee: 4,200.00 DKK

Status: Course is open for application

Semester: Spring 2024

Application deadline: 01/04/2024

Cancellation deadline: 15/04/2024

Course type: Classroom teaching

Start date: 29/04/2024

Administrator: Thilde Møller Risgaard

The course B244/09 Genome Engineering is being offered by the Graduate School of Health, Aarhus University, spring 2024.

Criteria for participation: University degree in medicine, dentistry, nursing, or master’s degree in other fields and/or postgraduate research fellows (PhD students and research-year medical students).

Recommended knowledge for participation (if any): Genetics, molecular biology, cell biology, and basic bioinformatics.

Aim: The aim of the course is to:

  1. Provide students with the state-of-the-art knowledge on CRISPR-Cas9 technology
  2. Introduce the different types of CRISPR gene editing tools (e.g. base editing, prime editing) and their applications
  3. Instruct how to design the best CRISPR gRNA with high on-target efficiency and low off-target effect – CRISPR data science
  4. Instruct how to design CRISPR and donor vectors for gene knockin, tagging
  5. Introduce CRISPR data science and high-throughput CRISPR computing
  6. Instruct students how to practically analyze and understand CRISPR editing data with TIDE, ICE and NGS
  7. Introduce the current progress in CRISPR gene therapy
  8. Introduce methods (both viral and non-viral) for CRISPR delivery
  9. Prepare PhD students with essential knowledge and tools for their gene editing projects

Learning outcomes: 
With the completion of this Genome Engineering course, PhD students are expected to

  1. Acquire a systematic knowledge on CRISPR gene editing technology, progresses, and applications
  2. Know how to design the CRISPR gRNA for gene knockout
  3. Learn how to design the donors for gene knockin
  4. Independently select the best CRISPR system and strategy for a gene editing project
  5. Independently analyze and interpret CRISPR gene editing results
  6. Know the concept, resources and potential of CRISPR data science
  7. Understand the progresses, but also remaining challenges, in CRISPR gene therapy

Workload: The full workload of the course is expected to be 60 hours.

Content: The course covers:

  1. The principle of CRISPR gene editing
  2. The different genetic manipulation tools derived from CRISPR-Cas9
  3. Base editing
  4. Prime editing
  5. Methods used for quantifying CRISPR editing efficiency
  6. Ways of delivery CRISPR vectors in vitro and/or in vivo: plasmid, RNP, lentivirus, AAV
  7. CRISPR data science – on-target activity and off-target effect prediction
  8. Group teaching on CRISPR vector design for gRNA and donor design for gene knockout, knockin, tagging and single nucleotide mutagenesis
  9. Group teaching on data analysis
  10. Introduction to CRISPR gene therapy

Instructors: Jan Gorodkin, Copenhagen University; Jacob Giehm Mikkelsen, Aarhus University; Rasmus O. Bak, Aarhus University; Lin Lin, Aarhus University

Venue: Aarhus University. To be announced.

Participation in the course is without cost for:

  • PhD students, Research Year students and Research Honours Programme students from Aarhus University
  • PhD students enrolled at partner universities of the Nordoc collaboration
  • PhD students from other institutions in the open market agreement for PhD courses

Course dates:

  • 29 April 2024 09:00 - 16:00
  • 30 April 2024 09:00 - 16:00
  • 01 May 2024 09:00 - 16:00
  • 02 May 2024 09:00 - 16:00
  • 03 May 2024 09:00 - 16:00