Course leader: Lene Niemann Nejsum
Graduate school: Faculty of Health
Graduate program: ClinFO
Course fee: 4,680.00 DKK
Status: Course is open for application
Semester: Spring 2024
Application deadline: 19/12/2023
Cancellation deadline: 02/01/2024
Course type: Classroom teaching
Start date: 16/01/2024
Administrator: Anne Fabricius
Day 4 and 5 are special
Please note that there will only be 2 hours per group on day 4 or 5 (23 and 24 January); it is not two whole days of teaching! The groups will be decided on course start.
Title: Introduction to Fluorescence Microscopy
is being offered by the Graduate School of Health, Aarhus University, 2024
Criteria for participation:
University degree in medicine, dentistry, nursing, or master’s degree in other fields and/or postgraduate research fellows (PhD students and research-year medical students).
You must submit a written application when signing up for the course. Please note, that everyone will be placed on waiting list and the seats will be given upon the approval of your application by the course leader.
The aim is to give the participants an overview of basic fluorescence microscopy techniques, sample preparation, image acquisition and imaging analysis.
The students will obtain basic knowledge of different fluorescence microscopy techniques, sample preparation, image acquisition and image analysis. This should enable students to prepare samples for fluorescence microscopy, choose the appropriate microscope setup, acquire images, analyze images and generate publication figures. This will enable students to participate in research projects involving fluorescence microscopy.
- Overview of basic light and fluorescence microscopy methods including white light microscopy, wide-field fluorescence microscopy and confocal microscopy as well as optics, cameras and photodetectors.
- Introduction to sample preparation of cells and paraffin embedded tissue sections for fluorescence microscopy (hands on).
- Introduction to image analysis using ImageJ Fiji; this includes working with multicolor images, background handling and fluorescence quantification (hands on) and
- Practical demonstration of wide-field fluorescence image acquisition using an inverted, automated microscope.
- There will be a mandatory home assignment equivalent to 6 h
Recommended knowledge for participation:
This is an introductory course, so no experience with fluorescence microscopy is necessary.
Head of course:
Lene Niemann Nejsum
Assistant Professor Frédéric H. Login, PhD student Benjamin Bach Green and PhD student Amalie Grønning
Time and date:
Note, on the 23-24/1, it will only be 2 h per student.
Venue: Bartholin Læsesal and adjacent laboratories. Forum AUH.
Participation in the course is without cost for:
- PhD students, Research Year students and Research Honours Programme students from Aarhus University
- PhD students enrolled at partner universities of the Nordoc collaboration
- PhD students from other institutions in the open market agreement for PhD courses
- 16 January 2024 08:30 - 16:00
- 17 January 2024 08:30 - 16:00
- 18 January 2024 08:30 - 16:00
- 23 January 2024 10:00 - 16:00
- 24 January 2024 10:00 - 16:00
- 01 February 2024 08:30 - 17:00